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Image Search Results
Journal: Open Biology
Article Title: A systematic investigation of production of synthetic prions from recombinant prion protein
doi: 10.1098/rsob.150165
Figure Lengend Snippet: Example positive wells from the matrix. ELISPOT wells containing spots with the characteristic visual appearance seen in prion bioassay by SCA were observed to increase successively over cell splits three to five. The matrix conditions that generated these positive findings are detailed next to the wells and were observed in the presence of copper ions at a 5 : 1 molar ratio with PrP and incubation at room temperature (RT) for 1 h (immediate) prior to inoculating the cells. These findings were not consistently reproducible.
Article Snippet: Spot counts (reporting PK-resistant PrP-positive cells; see below) were determined with a
Techniques: Enzyme-linked Immunospot, Bioassay, Generated, Incubation